Ⅰ. Seven joint tests for the Th1/Th2/Th17 subgroup
The levels of IL-2, IL-4, IL-6, IL-10, TNF-α、IFN-γ and IL-17A in patient samples were detected in vitro, and the immune status and inflammatory reaction were monitored.
Th1 type immune response: representative of cytokines IL-2, IFN-γ, TNF-α
Pro-inflammatory response, autoimmune response |
IL-2 |
Mediate the proliferation of activated T cells and promote the survival of T cells. Drives Th2 cell differentiation |
Mainly mediating cellular immunity, usually dealing with infections caused by viruses and certain bacteria, Th1 cells are the body's first line of defense against pathogens entering cells, and are also involved in the development of organ-specific autoimmunity. |
IFN-γ |
The main pro-inflammatory factor, activates macrophages, enhances their ability to phagocytic cells and destroy microorganisms |
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TNF-α |
Cooperate with IFN-γ to promote the sterilization of macrophages |
Anti-inflammatory and allergic reactions |
IL-4 |
Drives Th2 cell differentiation and induces B cells to produce IgE antibodies |
It mainly mediates fluid immunity and participates in clearing bacteria, toxins, allergens, parasites, etc. Th2 cells are responsible for stimulating the production of antibodies that respond to extracellular pathogens, and participate in autoimmune diseases and other chronic diseases. |
IL-6 |
Powerful pro-inflammatory effect; induce Th2 cell differentiation by promoting the production of IL-4; inhibit IFN-γ production and Th1 cell differentiation |
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IL-10 |
Strong anti-inflammatory effect, inhibit the differentiation of Th1 cell |
Th17 type immune response: representative cytokine IL-17A
Proinflammatory response, autoimmune response |
IL-17A |
Plays a key role in inflammation, autoimmune disease, and host defense |
It has the functions of Th1 and Th2, and controls the balance of Th1/Th2, which is very important in the homeostasis of the immune system. At the same time, it can also function in places where Th1 cells and Th2 cells are not responsible for fighting extracellular bacteria and mycoplasma. |
Ⅱ. Seven joint tests for the Th1/Th2/Th17 subgroup
1. For allergies or autoimmune diseases, the level of Th1/Th2/Th17 subgroup cytokines can be detected, the immune status can be checked, combined with clinical symptoms or other auxiliary examinations. And personalized treatment plans can be formulated to help balance the immune system and reduce the intensity of the autoimmune attack.
2. During pregnancy, the body will suppress Th1 type immune response, and Th2 type immune response dominates. The purpose is to reduce the mother's rejection of the fetus. IL-17 is a pro-inflammatory cytokine that can induce the expression of a variety of inflammatory mediators. The differentiation of a large number of Th17 cells will cause recurrent or inevitable abortion. Through the detection of Th1 / Th2 / Th17 subsets to judge the maternal immune status, given certain intervention measures, can help complete normal pregnancy.
3. Intestinal worm infection triggers a type 2 response, which promotes Th2 cell-mediated immunity. Th2 cells release a series of cytokines. These factors drive the effector mechanism and expand themselves through a positive feedback loop, thereby amplifying the response, and the immune system is overdone. Polarization will cause immune imbalance and lead to various diseases, so Th1 type response is activated and Th2 type immune response is suppressed. By detecting the representative cytokines of Th1/Th2, the immune status of the body can be judged, assisted in guiding intervention measures, and excessive deviation of the immune status of the body can be prevented.
4. In the early stage of rheumatoid arthritis, Th17 cells produce IL-17A in joints to stimulate synovial fibroblasts, osteoblasts and chondrocytes to release some inflammatory molecules. In the late stage, the Th1 response is dominant. Th1 cells activate macrophages through the production of IFN-γ, thereby participating in the inflammatory process. The patient’s cytokine content can be used to determine the patient’s disease course. Treatment according to the disease course can reduce overtreatment and avoid irreversible joint damage.
III.department application
Oncology, Infectious Diseases, Hematology, Respiratory, Nephrology, Rheumatology, ICU,etc.
