The contents of thyroid stimulating hormone, free triiodothyronine, free tetraiodothyronine, anti-thyroglobulin antibody and anti-thyroid peroxidase antibody in human serum samples were quantitatively detected. At present, chemiluminescence immunoassay is mostly used to detect the content of thyroid-stimulating hormone, free triiodothyronine, free tetraiodothyronine, anti-thyroglobulin antibody, and anti-thyroid peroxidase antibody.